Identification of a receptor for calcitonin gene-related peptides I and II in human cerebellum

Biochem Biophys Res Commun. 1988 Mar 30;151(3):1081-7. doi: 10.1016/s0006-291x(88)80476-5.


Receptor proteins for human calcitonin gene-related peptide (CGRP) have been demonstrated in the human cerebellum. Membrane homogenates were incubated with [125I] iodo CGRP-I and -II ([125I]CGRP). The bound radioligands were cross-linked to membrane proteins. Solubilized membrane proteins were separated by gel-electrophoresis. Autoradiography of the gels revealed specifically labeled ligand-protein complexes of Mr 17,500 and 54,000 for human CGRP-I and -II. Specific binding of [125I]CGRP-I and -II was only minimally affected by up to 0.5 microM salmon and human calcitonin. The affinity and specificity of CGRP-I and -II binding were the same in crude homogenates and in the purified binding proteins. In conclusion, indistinguishable binding proteins for [125I]CGRP-I and -II of 13,700 and 50,000 Da have been identified in the human cerebellum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Calcitonin Gene-Related Peptide
  • Cerebellum / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Membrane Proteins / analysis
  • Molecular Weight
  • Neuropeptides / metabolism*
  • Receptors, Calcitonin
  • Receptors, Cell Surface / metabolism*


  • Membrane Proteins
  • Neuropeptides
  • Receptors, Calcitonin
  • Receptors, Cell Surface
  • Calcitonin Gene-Related Peptide