Epidemiology of polyparasitism with Taenia solium, schistosomes and soil-transmitted helminths in the co-endemic village of Malanga, Democratic Republic of Congo

Acta Trop. 2017 Jul;171:186-193. doi: 10.1016/j.actatropica.2017.03.019. Epub 2017 Mar 20.

Abstract

Helminth co-infections are common in sub-Saharan Africa. However, little is known about the distribution and determinants of co-infections with Taenia solium taeniasis/cysticercosis. Building on a previous community-based study on human cysticercosis in Malanga village, we investigated co-infections with Taenia solium, soil-transmitted helminths (STHs) and Schistosoma spp and associated risk factors in a random subsample of 330 participants. Real time PCR assays were used to detect DNA of soil-transmitted helminths (STHs), T. solium and Schistosoma in stool samples and Schistosoma DNA in urine samples. Serum samples were tested for T. solium cysticercosis using the B158/B60 monoclonal antibody-based antigen ELISA. Bivariate analysis and logistic regression were applied to assess associations of single and co-infections with common risk factors (age, sex, area, hygiene) as well as pair wise associations between helminth species. Overall, 240 (72.7%) participants were infected with at least one helminth species; 128 (38.8%) harbored at least two helminth species (16.1% with STHs-Schistosoma, 14.5% with STHs-T. solium taeniasis/cysticercosis and 8.2% with Schistosoma-T. solium taeniasis/cysticercosis co-infections). No significant associations were found between Schistosoma-T. solium taeniasis/cysticercosis co-infection and any of the risk factors studied. Males (OR=2 (95%CI=1.1-5), p=0.03) and open defecation behavior (OR=3.8 (95%CI=1.1-6.5), p=0.04) were associated with higher odds of STHs-T. solium taeniasis/cysticercosis co-infection. Village districts that were found at high risk of T. solium taeniasis/cysticercosis were also at high risk of co-infection with STHs and T. solium taeniasis/cysticercosis (OR=3.2 (95%CI=1.1-7.8), p=0.03). Significant pair-wise associations were found between T. solium cysticerci and Necator americanus (OR=2.2 (95%CI=1.2-3.8), p<0.01) as well as Strongyloides stercoralis (OR=2.7 (95%CI=1.1-6.5), p=0.02). These findings show that co-infections with T. solium are common in this polyparasitic community in DRC. Our results on risk factors of helminth co-infections and specific associations between helminths may contribute to a better integration of control within programmes that target more than one NTD.

Keywords: Co-infection; Democratic Republic of Congo; Helminths; Polyparasitism; Schistosoma; Soil-transmitted helminths; Taenia solium.

MeSH terms

  • Animals
  • Antibodies, Helminth / blood
  • Coinfection*
  • Congo
  • Cysticercosis / epidemiology*
  • Cysticercosis / parasitology
  • Cysticercus
  • Democratic Republic of the Congo / epidemiology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Risk Factors
  • Schistosoma
  • Schistosomiasis / epidemiology*
  • Schistosomiasis / parasitology
  • Soil / parasitology*
  • Taenia solium
  • Taeniasis / epidemiology*
  • Taeniasis / parasitology

Substances

  • Antibodies, Helminth
  • Soil