Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification

Monoclon Antib Immunodiagn Immunother. 2017 Apr;36(2):68-71. doi: 10.1089/mab.2016.0052. Epub 2017 Mar 24.


Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

Keywords: RAP tag; affinity tag; monoclonal antibody; podoplanin; protein purification.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry*
  • Antibody Affinity
  • Antibody Specificity
  • CHO Cells
  • Cricetulus
  • ErbB Receptors / genetics
  • ErbB Receptors / immunology
  • ErbB Receptors / isolation & purification*
  • Gene Expression
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology*
  • Mice
  • Peptides / chemistry
  • Peptides / immunology*
  • Rats
  • Staining and Labeling / methods*


  • Antibodies, Monoclonal
  • Membrane Glycoproteins
  • Pdpn protein, rat
  • Peptides
  • ErbB Receptors