The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V

Carbohydr Res. 1988 Mar 1;173(2):263-72. doi: 10.1016/s0008-6215(00)90822-x.


Incubation of the trisaccharide acceptor, beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp-O( CH2)8CO2Me with sonicates of Rous sarcoma-transformed baby-hamster kidney cells, which contain N-acetylglucosaminyltransferase V activity, resulted in the production of beta-D-GlcpNAc-(1----2)-[beta-D-GlcpNAc-(1----6)]-alpha-D-Manp-(1- ---6)- beta-D-Manp-O(CH2)8CO2Me (4). The product of the enzymic reaction was identified by comparison of its 1H-n.m.r. spectrum with that of authentic 4 whose chemical synthesis is also described.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Avian Sarcoma Viruses / enzymology*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cell Line
  • Cell Transformation, Viral
  • Cricetinae
  • Indicators and Reagents
  • Kidney
  • Molecular Sequence Data
  • Substrate Specificity
  • Trisaccharides / metabolism*


  • Indicators and Reagents
  • Trisaccharides