Sub-attomolar electrochemical measurement of DNA hybridization based on the detection of high coverage biobarcode latex labels at PNA-modified screen printed electrodes

Talanta. 2017 May 15;167:14-20. doi: 10.1016/j.talanta.2017.01.094. Epub 2017 Feb 1.

Abstract

We have constructed biobarcode labels based on 468nm diameter latex spheres. Modification with polyallylamine and then glutaraldehyde was used to attach a high DNA loading, consisting of aminated probe DNA (approx. 1.01×102 molecules per sphere) and biobarcode DNA (approx. 1.66×104 molecules per sphere). Detection of the biobarcodes was performed by application of a Ag enhancer solution, causing association of the Ag+ ions with the phosphate groups of the DNA. The deposited Ag was detected by differential pulse voltammetry. A 30 mer sequence from the BL21 strain of E. coli was detected with an LOD of 2.6fM (calibration range 10 aM to 0.1pM, r2=0.91, n=45). The LOD was lowered to 0.56aM (calibration range 100zM to 0.1nM, r2=0.991, n=50) by utilizing a sandwich assay with PNA-modified screen printed electrodes, which lowered the Ag background current. The sandwich assay platform was used to calibrate E. coli strain BL2(DE3) with an LOD of 17.0 CFU mL-1 (calibration range 10 to 106 CFU mL-1, r2=0.99, n=33) with good discrimination against Salmonella.

Keywords: E. coli; Electrochemical; Nucleic acid; PNA; Voltammetry.

MeSH terms

  • DNA, Bacterial / analysis*
  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / genetics
  • Electrochemistry / instrumentation*
  • Electrodes
  • Escherichia coli / genetics
  • Latex / chemistry*
  • Nucleic Acid Hybridization
  • Peptide Nucleic Acids / chemistry*
  • Printing

Substances

  • DNA, Bacterial
  • Latex
  • Peptide Nucleic Acids