Design and synthesis of affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase

Bioorg Med Chem. 2017 Jan 1;25(1):116-125. doi: 10.1016/j.bmc.2016.10.017. Epub 2016 Oct 15.

Abstract

Arachidonic acid (AA) is converted to biologically active metabolites by different pathways, one of the most important of which is initiated by 5-lipoxygenase (5-LO). 5-Hydroxyeicosatetraenoic acid (5-HETE), although possessing only weak biological activity itself, is oxidized to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent chemoattractant for eosinophils and neutrophils. Our main goal is to determine how the biosynthesis of 5-oxo-ETE is regulated and to determine its pathophysiological roles. To achieve this task, we designed and synthesized affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase (5-HEDH), the enzyme responsible for the formation of 5-oxo-ETE.

Keywords: 5-HEDH; 5-HETE; 5-Oxo-ETE; Affinity chromatography ligand; Asthma; Eosinophil; Neutrophil.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / isolation & purification*
  • Alcohol Oxidoreductases / metabolism
  • Arachidonic Acids / metabolism
  • Cell Line
  • Chromatography, Affinity / methods*
  • Humans
  • Hydroxyeicosatetraenoic Acids / metabolism
  • Ligands
  • Neutrophils / metabolism

Substances

  • Arachidonic Acids
  • Hydroxyeicosatetraenoic Acids
  • Ligands
  • 5-oxo-6,8,11,14-eicosatetraenoic acid
  • 5-hydroxy-6,8,11,14-eicosatetraenoic acid
  • Alcohol Oxidoreductases
  • 5-hydroxyeicosanoid dehydrogenase