The Drosophila ets-2 gene: molecular structure, chromosomal localization, and developmental expression

Dev Biol. 1988 May;127(1):45-53. doi: 10.1016/0012-1606(88)90187-x.

Abstract

The cellular homologs of the ets gene from the avian erythroblastosis retrovirus E26 have been studied in chickens, humans, mice, and cats. In this report a further evolutionary step is taken by isolating and characterizing a Drosophila ets-related genomic clone. Sequence analysis of this clone has shown it to contain the 3' end of the v-ets gene, called ets-2, corresponding to the last two exons of chicken ets. The predicted amino acid sequence was found to have over 90% homology when compared to that of v-ets. This is the highest level of conservation observed for any previously characterized Drosophila oncogene homolog. Expression of the ets-2 gene occurs throughout development, but is highest during the embryonic and pupal stages. By in situ hybridization, the ets-2 chromosomal position was determined to be 58A/B which corresponds to no known phenotypic mutant. As this is a highly conserved gene, the Drosophila model system should prove useful for the determination of the ets gene function.

Publication types

  • Comparative Study

MeSH terms

  • Alpharetrovirus / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Chromosome Mapping
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Exons
  • Gene Expression Regulation*
  • Introns
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Proto-Oncogenes*
  • Pupa / metabolism
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Recombinant
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M20408