Adaptation of Hepatitis A Virus to High Titre Growth in Diploid and Permanent Cell Cultures

Med Microbiol Immunol. 1988;177(2):91-100. doi: 10.1007/BF00189530.


A hepatitis A virus isolate originally obtained from the feces of a clinically ill patient and passaged in diploid human embryonic kidney and lung cells was adapted to grow in MRC-5, Cercopithecus aethiops muscle and in Vero cells. Three different adaptation methods were applied. Either method proved to be suitable to finally give high virus titres of cell-bound as well as cell-free virus in the supernatant of infected cultures during 10 to 15 passages. An easily performable immunoperoxidase staining method was used for the titration of hepatitis A virus in microtitre plates. Cytopathogenic changes in MRC-5 cell cultures infected with fully adapted virus are described.

MeSH terms

  • Cells, Cultured
  • Diploidy
  • Hepatovirus / growth & development*
  • Kidney
  • Lung
  • Muscles
  • Virus Cultivation*