Insertional mutagenesis of the myc locus by a LINE-1 sequence in a human breast carcinoma

Nature. 1988 May 5;333(6168):87-90. doi: 10.1038/333087a0.

Abstract

The proto-oncogene c-myc is the cellular homologue of the transforming sequence carried by the avian myelocytomastosis virus MC29. A growing body of evidence implicates structural and functional alterations in and around proto-oncogenes such as c-myc in tumorogenesis. Here we report that comparison of the structure of myc from a ductal adenocarcinoma of the breast and from normal breast tissue of the same patient (Sc) revealed a tumour-specific rearrangement of one myc locus and amplification of the other myc locus. (For myc reviews see refs 1-4; for myc involvement in breast neoplasia see refs 5-7.) Within the second intron of the rearranged locus was a non-myc sequence with nearly complete homology to a long interspersed repetitive element (a LINE-1 sequence or L1). In this case, the L1 sequence has functioned as a mobile genetic element to produce a somatic mutation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Breast Neoplasms / genetics*
  • DNA Restriction Enzymes
  • DNA Transposable Elements
  • Female
  • Humans
  • Molecular Sequence Data
  • Mutation*
  • Nucleotide Mapping
  • Proto-Oncogenes*

Substances

  • DNA Transposable Elements
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X07803
  • GENBANK/Y00304