Vascular basement membrane alterations and β-amyloid accumulations in an animal model of cerebral small vessel disease

Clin Sci (Lond). 2017 May 1;131(10):1001-1013. doi: 10.1042/CS20170004. Epub 2017 Mar 27.


Non-amyloid cerebral small vessel disease (CSVD) and cerebral amyloid angiopathy (CAA) may be interrelated through the damaged basement membranes (BMs) and extracellular matrix changes of small vessels, resulting in a failure of β-amyloid (Aβ) transport and degradation. We analyzed BM changes and the pattern of deposition of Aβ in the walls of blood vessels in spontaneously hypertensive stroke-prone rats (SHRSP), a non-transgenic CSVD model. In 45 SHRSP and 38 Wistar rats aged 18 to 32 weeks: (i) the percentage area immunostained for vascular collagen IV and laminin was quantified; (ii) the capillary BM thickness as well as endothelial and pericyte pathological changes were analysed using transmission electron microscopy (TEM); and (iii) the presence of vascular Aβ was assessed. Compared with controls, SHRSP exhibited a significantly higher percentage area immunostained with collagen IV in the striatum and thalamus. SHRSP also revealed an age-dependent increase of the capillary BM thickness and of endothelial vacuoles (caveolae) within subcortical regions. Endogenous Aβ deposits in the walls of small blood vessels were observed in the cortex (with the highest incidence found within fronto-parietal areas), striatum, thalamus and hippocampus. Vascular β-amyloid accumulations were frequently detected at sites of small vessel wall damage. Our data demonstrate changes in the expression of collagen IV and of the ultrastructure of BMs in the small vessels of SHRSP. Alterations are accompanied by vascular deposits of endogenous Aβ. Impaired β-amyloid clearance along perivascular and endothelial pathways and failure of extracellular Aβ degradation may be the key mechanisms connecting non-amyloid CSVD and CAA.

Keywords: cerebral amyloid angiopathy; cerebral small vessel disease; perivascular Aβ drainage; spontaneously hypertensive animal model.

MeSH terms

  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Basement Membrane / metabolism*
  • Cerebral Amyloid Angiopathy / metabolism
  • Cerebral Small Vessel Diseases / metabolism*
  • Disease Models, Animal
  • Humans
  • Microvessels / metabolism*
  • Rats
  • Rats, Inbred SHR
  • Rats, Wistar


  • Amyloid beta-Peptides