In Vivo Imaging of Budding Yeast Meiosis

Michael G Pollard; Jennifer C Fung

doi:10.1007/978-1-4939-6340-9_9

In Vivo Imaging of Budding Yeast Meiosis

Methods Mol Biol. 2017:1471:175-186. doi: 10.1007/978-1-4939-6340-9_9.

Authors

Michael G Pollard¹, Jennifer C Fung^{2

3}

Affiliations

¹ Department of Obstetrics, Gynecology and Reproductive Sciences, Center of Reproductive Sciences, University of California, San Francisco, CA, 94143, USA.
² Department of Obstetrics, Gynecology and Reproductive Sciences, Center of Reproductive Sciences, University of California, San Francisco, CA, 94143, USA. jennifer.fung@ucsf.edu.
³ Fung Lab, University of California, San Francisco, GH N416, 600 16th St., San Francisco, CA, 94158, USA. jennifer.fung@ucsf.edu.

PMID: 28349396
DOI: 10.1007/978-1-4939-6340-9_9

Abstract

Tracking biological events in living cells provides kinetic information about biological processes that can be missed in more traditional methods using fixed samples at designated time intervals. Here we describe a methodology for in vivo fluorescence microscopy of yeast cells undergoing meiosis. This method allows tracking of individual cells over extended periods of time through every stage of the meiotic transformation while minimizing phototoxicity and sustaining conditions that support meiotic growth.

Keywords: In vivo fluorescence microscopy; Live imaging; Meiosis; Yeast.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Meiosis*
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods*
Molecular Imaging / methods*
Saccharomycetales / genetics
Saccharomycetales / physiology*

Abstract

Publication types

MeSH terms

Grants and funding