Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Methods Mol Biol. 2017;1591:177-194. doi: 10.1007/978-1-4939-6931-9_13.

Abstract

Lymphocyte recruitment to different organs, and even alternate anatomical regions within the same organ, is differentially regulated. Key combinations of adhesion molecules and chemokines govern compartmentalization, and these can change depending upon the nature and duration of tissue injury. We are interested in understanding lymphocyte recruitment to the liver during human disease, and thus need models of the liver inflammatory milieu that are as representative as possible. Here we describe the use of precision cut liver slices as models of disease.

Keywords: Antibody; Human; Inflammation; Liver; Lymphocyte; Organ culture.

MeSH terms

  • Animals
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / metabolism
  • Cell Movement / physiology*
  • Chemokines / metabolism
  • Humans
  • Liver / cytology*
  • Lymphocytes / cytology*
  • Lymphocytes / metabolism
  • Lymphocytes / physiology*
  • Organ Culture Techniques / methods

Substances

  • Cell Adhesion Molecules
  • Chemokines