cDNA cloning and amino acid sequence of human brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase

Biochem Biophys Res Commun. 1988 Apr 29;152(2):837-42. doi: 10.1016/s0006-291x(88)80114-1.

Abstract

A cDNA of 1762 base pairs was obtained from a cDNA library of human brain by immunoscreening, and the nucleotide sequence of the cDNA was determined. The complete amino acid sequence of human 2',3'-cyclic-nucleotide 3'-phosphodiesterase was deduced from the nucleotide sequence of the cDNA. Human enzyme was found to contain 401 amino acids including initiation methionine and have a molecular weight of 45,098. RNA blot hybridization revealed a single mRNA band at the position of about 3000 bases. DNA blot hybridization suggested that a single-copy 2',3'-cyclic-nucleotide 3'-phosphodiesterase gene exists per haploid genome.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2',3'-Cyclic-Nucleotide Phosphodiesterases / genetics*
  • 2',3'-Cyclic-Nucleotide Phosphodiesterases / isolation & purification
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology*
  • Cattle
  • Cloning, Molecular*
  • DNA / isolation & purification*
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Protein Biosynthesis
  • Rats

Substances

  • DNA
  • 2',3'-Cyclic-Nucleotide Phosphodiesterases

Associated data

  • GENBANK/M19650