Cryobanking Pluripotent Stem Cells

Methods Mol Biol. 2017;1590:151-164. doi: 10.1007/978-1-4939-6921-0_11.


Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.

Keywords: Banking; Control rate freezing; Cryopreservation; Human pluripotent stem cells; Vitrification.

MeSH terms

  • Cell Culture Techniques / methods
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Cryopreservation / methods
  • Cryoprotective Agents / pharmacology
  • Freezing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / drug effects
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / drug effects
  • Vitrification


  • Cryoprotective Agents