Culturing and Cryobanking Human Neural Stem Cells

Methods Mol Biol. 2017:1590:199-206. doi: 10.1007/978-1-4939-6921-0_15.


The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

Keywords: Cryopreservation; Culture; Expansion; Freezing; Human neural stem cells; Passaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Specimen Banks
  • Cell Culture Techniques / methods
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Cryopreservation / methods
  • Humans
  • Neural Stem Cells / cytology*
  • Neurogenesis / physiology
  • Neuroglia / cytology
  • Neurons / cytology