BcefI, a new type IIS restriction endonuclease

P Venetianer; A Orosz

doi:10.1093/nar/16.7.3053

BcefI, a new type IIS restriction endonuclease

Nucleic Acids Res. 1988 Apr 11;16(7):3053-60. doi: 10.1093/nar/16.7.3053.

Authors

P Venetianer¹, A Orosz

Affiliation

¹ Institute of Biochemistry, Biological Research Center, Szeged, Hungary.

Abstract

A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.

MeSH terms

Bacillus cereus / enzymology*
Base Sequence
Cloning, Molecular / methods
DNA Restriction Enzymes / isolation & purification*
Deoxyribonucleases, Type II Site-Specific*
Molecular Sequence Data
Plasmids
Sequence Homology, Nucleic Acid

Substances

DNA Restriction Enzymes
CGCG-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific