A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri

J Microbiol Methods. 2017 Jun;137:19-24. doi: 10.1016/j.mimet.2017.03.015. Epub 2017 Mar 27.

Abstract

Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.

Keywords: Fluorescence; Granulocyte; Inflammation; Probiotic.

MeSH terms

  • Aminoglycosides / chemistry
  • Animals
  • Eosinophils / microbiology*
  • Escherichia coli / drug effects
  • Flow Cytometry / methods*
  • Granulocytes / microbiology
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / microbiology
  • Kanamycin / pharmacology
  • Lactobacillus reuteri / isolation & purification
  • Lactobacillus reuteri / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Probiotics / pharmacokinetics*

Substances

  • Aminoglycosides
  • Kanamycin