In Vivo Model of Small Intestine

Methods Mol Biol. 2017;1597:229-245. doi: 10.1007/978-1-4939-6949-4_17.

Abstract

The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

Keywords: Bioengineered small intestine; Human intestinal organoids; In vivo model; Kidney capsule; Mesentery; Pluripotent stem cells; Transplantation.

MeSH terms

  • Animals
  • Cell Differentiation / physiology
  • Female
  • Humans
  • Intestine, Small / cytology*
  • Male
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Models, Biological
  • Organoids / cytology
  • Pluripotent Stem Cells / cytology