Automatic tracing of ultra-volumes of neuronal images

doi:10.1038/nmeth.4233

. 2017 Mar 31;14(4):332-333.

doi: 10.1038/nmeth.4233.

Automatic tracing of ultra-volumes of neuronal images

Hanchuan Peng¹, Zhi Zhou¹, Erik Meijering², Ting Zhao³, Giorgio A Ascoli⁴, Michael Hawrylycz¹

Affiliations

¹ Allen Institute for Brain Science, Seattle, Washington, USA.
² Erasmus University Medical Center Rotterdam, Rotterdam, the Netherlands.
³ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
⁴ Krasnow Institute for Advanced Study, George Mason University, Fairfax, Virginia, USA.

PMID: 28362437
PMCID: PMC7199653
DOI: 10.1038/nmeth.4233

Automatic tracing of ultra-volumes of neuronal images

Hanchuan Peng et al. Nat Methods. 2017.

. 2017 Mar 31;14(4):332-333.

doi: 10.1038/nmeth.4233.

Authors

Hanchuan Peng¹, Zhi Zhou¹, Erik Meijering², Ting Zhao³, Giorgio A Ascoli⁴, Michael Hawrylycz¹

Affiliations

¹ Allen Institute for Brain Science, Seattle, Washington, USA.
² Erasmus University Medical Center Rotterdam, Rotterdam, the Netherlands.
³ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
⁴ Krasnow Institute for Advanced Study, George Mason University, Fairfax, Virginia, USA.

PMID: 28362437
PMCID: PMC7199653
DOI: 10.1038/nmeth.4233

No abstract available

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Conflict of interest statement

Competing interests

The authors declare no competing financial interests.

Figures

**Figure 1:. Workflow of UltraTracer for tracing a large 3D image volume.**
(a) 3D confocal image stack of a Lucifer-Yellow-labeled human pyramidal neuron. The voxel size is 0.18 × 0.18 × 0.5 μm, and the overlaid grid (black lines) indicates how the image volume is subdivided into uniform tiles. (b) UltraTracer first traces the subarea containing the soma and then detects the neuron terminal tips in the reconstruction, and adaptively explores and traces neighboring subareas. Green boxes indicate terminal tips detected in tracing a subarea. (c) Final reconstruction produced by UltraTracer, with zooms of two parts for detailed visualization.

See this image and copyright information in PMC

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References

1. Helmstaedter M Nat. Methods 10, 501–507 (2013). - PubMed
1. Acciai L, Soda P & Iannello G Neuroinformatics 4, 353–367 (2016). - PubMed
1. Peng H et al. Neuron 87, 252–256 (2015). - PMC - PubMed
1. Zhao T et al. Neuroinformatics 9, 247–261 (2011). - PMC - PubMed
1. Wu J et al. Neuroimage 87, 199–208 (2014). - PubMed

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[1] Helmstaedter M Nat. Methods 10, 501–507 (2013). - PubMed

[2] Helmstaedter M Nat. Methods 10, 501–507 (2013). - PubMed

[3] Acciai L, Soda P & Iannello G Neuroinformatics 4, 353–367 (2016). - PubMed

[4] Acciai L, Soda P & Iannello G Neuroinformatics 4, 353–367 (2016). - PubMed

[5] Peng H et al. Neuron 87, 252–256 (2015). - PMC - PubMed

[6] Peng H et al. Neuron 87, 252–256 (2015). - PMC - PubMed

[7] Zhao T et al. Neuroinformatics 9, 247–261 (2011). - PMC - PubMed

[8] Zhao T et al. Neuroinformatics 9, 247–261 (2011). - PMC - PubMed

[9] Wu J et al. Neuroimage 87, 199–208 (2014). - PubMed

[10] Wu J et al. Neuroimage 87, 199–208 (2014). - PubMed

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Automatic tracing of ultra-volumes of neuronal images

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Automatic tracing of ultra-volumes of neuronal images

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Conflict of interest statement

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