Effect of selenylation modification on antitumor activity of peptidoglycan from Lactobacillus acidophilus

Jiayi He; Zhen Wu; Daodong Pan; Yuxing Guo; Xiaoqun Zeng

doi:10.1016/j.carbpol.2017.02.031

Effect of selenylation modification on antitumor activity of peptidoglycan from Lactobacillus acidophilus

Carbohydr Polym. 2017 Jun 1:165:344-350. doi: 10.1016/j.carbpol.2017.02.031. Epub 2017 Feb 14.

Authors

Jiayi He¹, Zhen Wu², Daodong Pan³, Yuxing Guo⁴, Xiaoqun Zeng¹

Affiliations

¹ Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China.
² Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China. Electronic address: woodsen@163.com.
³ Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China; Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China. Electronic address: daodongpan@163.com.
⁴ Department of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China.

PMID: 28363558
DOI: 10.1016/j.carbpol.2017.02.031

Abstract

Selenium is an essential trace element for human with the antitumor properties. In the present study, the peptidoglycan (PG) derived from Lactobacillus acidophilus was modified by selenylation with the HNO₃-Na₂SeO₃ method. Reaction temperature, reaction duration and the selenide content were optimized according to orthogonal design of three-factors. In addition, the molecular structure of selenizing peptidoglycan (Se-PG) was determined by infrared spectroscopy analysis. Furthermore, the antitumor activity of Se-PG was also investigated in HT-29 cells. The results showed that Se-PG exerted a greater antitumor activity than non-modified PG in a dose-dependent manner. These findings indicated that selenylation modification can enhance the antitumor activity of PG, and Se-PG could achieve its potential in antitumor activity.

Keywords: 5-Fluorouracil (PubChem CID:3385); Antitumor activity; Fluorescein isothiocyanate (PubChem CID: 18730); HT-29 cells; Peptidoglycan; Peptidoglycan (PubChem CID:3035036); Propidium iodide (PubChem CID: 104981); Selenylation modification; Trichloroacetic acid (PubChem CID: 6421).

MeSH terms

Antineoplastic Agents / pharmacology*
Cell Wall
HT29 Cells
Humans
Lactobacillus acidophilus / chemistry*
Peptidoglycan / pharmacology*
Selenium*

Substances

Antineoplastic Agents
Peptidoglycan
Selenium