Fyn regulates multipolar-bipolar transition and neurite morphogenesis of migrating neurons in the developing neocortex

Neuroscience. 2017 Jun 3;352:39-51. doi: 10.1016/j.neuroscience.2017.03.032. Epub 2017 Mar 29.


Fyn is a non-receptor protein tyrosine kinase that belongs to Src family kinases. Fyn plays a critical role in neuronal migration, but the mechanism remains unclear. Here, we reported that suppression of Fyn expression in mouse cerebral cortex led to migration defects of both early-born and late-born neurons. Morphological analysis showed that loss of Fyn function impaired multipolar-bipolar transition of newly generated neurons and neurite formation in the early phase of migration. Moreover, Fyn inhibition increased the length of leading process and decreased the branching number of the migrating cortical neurons. Together, these results indicate that Fyn controls neuronal migration by regulating the cytoskeletal dynamics and multipolar-bipolar transition of newly generated neurons during cortical development.

Keywords: Fyn; RNAi; in utero electroporation; neuronal migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Movement / physiology*
  • Embryo, Mammalian
  • Gene Expression Regulation, Developmental / genetics
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HEK293 Cells
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Neocortex* / cytology
  • Neocortex* / embryology
  • Neocortex* / growth & development
  • Nerve Tissue Proteins / metabolism
  • Neurites / metabolism*
  • Neurogenesis / genetics
  • Neurogenesis / physiology*
  • Neurons / cytology*
  • Neurons / physiology
  • POU Domain Factors / metabolism
  • Proto-Oncogene Proteins c-fyn / genetics
  • Proto-Oncogene Proteins c-fyn / metabolism*
  • Transduction, Genetic
  • Transfection


  • Nerve Tissue Proteins
  • POU Domain Factors
  • Pou3f2 protein, mouse
  • Green Fluorescent Proteins
  • Fyn protein, mouse
  • Proto-Oncogene Proteins c-fyn