The bacteriophage lambda cohesive end site: isolation of spacing/substitution mutations that result in dependence on Escherichia coli integration host factor

Mol Gen Genet. 1988 Apr;212(1):157-65. doi: 10.1007/BF00322459.


Substitution, insertion and deletion mutations have been constructed at the XmnI restriction site in cos lambda. The XmnI site is located between cosB, the site where terminase binds lambda DNA; and cosN, the site where terminase introduces staggered nicks to generate cohesive ends. Substitution mutations and deletion of a base pair (a -1 change) do not obviously affect lambda growth and DNA packaging. Changes of -2, +2 and -3 render lambda unable to grow on host cells lacking integration host factor (IHF). The -3 mutant has a reduced burst size in IHF+ cells, due to a defect in the initiation of packaging. A -7 deletion mutation is lethal. Models for the basis of these mutational effects are discussed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacteriophage lambda / genetics*
  • Base Sequence
  • Chromosome Deletion
  • DNA Restriction Enzymes
  • DNA-Binding Proteins / genetics*
  • Escherichia coli / genetics*
  • Genes, Viral*
  • Integration Host Factors
  • Lysogeny
  • Molecular Sequence Data
  • Mutation*


  • Bacterial Proteins
  • DNA-Binding Proteins
  • Integration Host Factors
  • DNA Restriction Enzymes