Transformation of Balb 3T3 cells by the BNLF-1 gene of Epstein-Barr virus

Oncogene. 1988 May;2(5):461-7.


The BNLF-1 protein is the only non-nuclear Epstein-Barr virus (EBV) encoded protein that has been detected in B-lymphocytes immortalized by EBV. We demonstrate that the BNLF-1 gene induces anchorage-independent growth and tumorigenic transformation of the murine cell-line, Balb/3T3. This demonstration extends the earlier observation that the BNLF-1 gene can transform Rat-1 cells. In addition we find that the BNLF-1 protein is located in the particulate fraction of cells, is phosphorylated, and is turned over with a half-life of 2.0 to 3.5 h in the BNLF-1 transformed Balb/3T3 cells, just as it is in EBV-genome-positive B-cell-lines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Division
  • Cell Line
  • Cell Transformation, Viral*
  • Genes, Viral*
  • Herpesvirus 4, Human / genetics*
  • Mice
  • Oncogene Proteins, Viral / physiology*
  • Phosphoproteins / physiology*
  • Subcellular Fractions / metabolism
  • Viral Matrix Proteins*


  • EBV-associated membrane antigen, Epstein-Barr virus
  • Oncogene Proteins, Viral
  • Phosphoproteins
  • Viral Matrix Proteins