Background: Sogatella furcifera is an important phloem sap-sucking and plant virus-transmitting migratory insect of rice. Because of its high reproductive potential, dispersal capability and transmission of plant viral diseases, S. furcifera causes considerable damage to rice grain production and has great economical and agricultural impacts. Comprehensive studies into ecological aspects and virus-host interactions of S. furcifera have been limited because of the lack of a well-assembled genome sequence.
Findings: A total of 241.3 Gb of raw reads from the whole genome of S. furcifera were generated by Illumina sequencing using different combinations of mate-pair and paired-end libraries from 17 insert libraries ranging between 180 bp and 40 kbp. The final genome assembly (0.72 Gb), with average N50 contig size of 70.7 kb and scaffold N50 of 1.18 Mb, covers 98.6 % of the estimated genome size of S. furcifera . Genome annotation, assisted by eight different developmental stages (embryos, 1 st -5 th instar nymphs, 5-day-old adults and 10-day-old adults), generated 21 254 protein-coding genes, which captured 99.59 % (247/248) of core CEGMA genes and 91.7 % (2453/2675) of BUSCO genes.
Conclusions: We report the first assembled and annotated whole genome sequence and transcriptome of S. furcifera . The assembled draft genome of S. furcifera will be a valuable resource for ecological and virus-host interaction studies of this pest.
Keywords: Annotation; Assembly; Genomics; Sogatella furcifera genome.
© The Author 2017. Published by Oxford University Press.