Neuroprotective properties of Madecassoside from Centella asiatica after hypoxic-ischemic injury

Pak J Pharm Sci. 2016 Nov;29(6):2047-2051.

Abstract

Madecassoside is one of increasingly used constituent of Centella asiatica, a frequently prescribed crude drug in South eastern Asia and China for wound healing. In the present experiment, it exposes the neuroprotective nature of Madecassoside in GT1-7 cell lines, further, which the antioxidant activities are performed. The cellular toxicity was assessed using 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay with increased cell viability with IC50 2.5µg/ml. the regulation of antioxidant levels showed changes in madecassoside treated cell lysate viz., SOD assay. Also, the antioxidative assays confirmed the negligible cellular damage caused to the GT1-7 cell lines. Hence, the results advocate that the current antioxidant and antitumor activity be justified by the high concentration of phenolic constituents, primarily the triterpene present in the C. asiatica.

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Antioxidants / toxicity
  • Biphenyl Compounds / chemistry
  • Cell Line
  • Cell Survival / drug effects
  • Centella / chemistry*
  • Dose-Response Relationship, Drug
  • Hypoxia-Ischemia, Brain / metabolism
  • Hypoxia-Ischemia, Brain / pathology
  • Hypoxia-Ischemia, Brain / prevention & control*
  • L-Lactate Dehydrogenase / metabolism
  • Mice
  • Neurons / drug effects*
  • Neurons / metabolism
  • Neurons / pathology
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Neuroprotective Agents / toxicity
  • Phytotherapy
  • Picrates / chemistry
  • Plants, Medicinal
  • Superoxide Dismutase / metabolism
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification
  • Triterpenes / pharmacology*
  • Triterpenes / toxicity

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Centella asiatica extract
  • Neuroprotective Agents
  • Picrates
  • Triterpenes
  • madecassoside
  • 1,1-diphenyl-2-picrylhydrazyl
  • L-Lactate Dehydrogenase
  • Superoxide Dismutase