Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus

Mol Microbiol. 1988 Mar;2(2):289-92. doi: 10.1111/j.1365-2958.1988.tb00030.x.


Sections of a cloned 27 kb segment of chromosomal DNA, associated with resistance to four antimicrobial agents in a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA), were tested for their ability to determine resistance when transformed into a sensitive laboratory strain of S. aureus. This was achieved by inserting the sections into a newly constructed shuttle vector, amplifying the recombinant DNA in E. coli, and transforming protoplasts of the sensitive S. aureus strain. Two sections of the cloned DNA were found to determine resistance separately to mercuric ion and to tetracycline, in both S. aureus and Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomes, Bacterial / physiology
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Drug Resistance, Microbial / genetics
  • Genes, Bacterial*
  • Mercury / pharmacology*
  • Methicillin / pharmacology*
  • Nucleotide Mapping
  • Repetitive Sequences, Nucleic Acid
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Tetracycline Resistance / genetics*


  • DNA Restriction Enzymes
  • Mercury
  • Methicillin