Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue

Vahid Serpooshan; Pu Chen; Haodi Wu; Soah Lee; Arun Sharma; Daniel A Hu; Sneha Venkatraman; Adarsh Venkataraman Ganesan; Osman Berk Usta; Martin Yarmush; Fan Yang; Joseph C Wu; Utkan Demirci; Sean M Wu

doi:10.1016/j.biomaterials.2017.03.037

Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue

Biomaterials. 2017 Jul:131:47-57. doi: 10.1016/j.biomaterials.2017.03.037. Epub 2017 Mar 28.

Authors

Vahid Serpooshan¹, Pu Chen², Haodi Wu¹, Soah Lee³, Arun Sharma¹, Daniel A Hu¹, Sneha Venkatraman⁴, Adarsh Venkataraman Ganesan⁵, Osman Berk Usta⁶, Martin Yarmush⁷, Fan Yang⁸, Joseph C Wu⁹, Utkan Demirci¹⁰, Sean M Wu¹¹

Affiliations

¹ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
² Bio-Acoustic MEMS in Medicine (BAMM) Lab, Department of Radiology, Stanford University School of Medicine, Canary Center for Early Cancer Detection, Stanford, CA, USA; Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan, China; Institute of Model Animal of Wuhan University, Wuhan, China.
³ Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
⁴ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Biology Program, California State University Channel Islands, Camarillo, CA, USA.
⁵ The Nanoscience Centre, University of Cambridge, Cambridge, United Kingdom.
⁶ Center for Engineering in Medicine at Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, 51 Blossom St., Boston 02144, MA, USA.
⁷ Center for Engineering in Medicine at Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, 51 Blossom St., Boston 02144, MA, USA; Department of Biomedical Engineering, Rutgers University, 599 Taylor Rd, Piscataway 08854, NJ, USA.
⁸ Department of Orthopaedic Surgery, Stanford, CA, USA; Department of Bioengineering, Stanford University, Stanford, CA, USA.
⁹ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Institute of Stem Cell Biology and Regenerative Medicine, Stanford, CA, USA; Department of Medicine, Division of Cardiovascular Medicine, Stanford University, Stanford, CA, USA.
¹⁰ Bio-Acoustic MEMS in Medicine (BAMM) Lab, Department of Radiology, Stanford University School of Medicine, Canary Center for Early Cancer Detection, Stanford, CA, USA; Department of Electrical Engineering (by Courtesy), Stanford University School of Engineering, Stanford, CA, USA. Electronic address: utkan@stanford.edu.
¹¹ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Institute of Stem Cell Biology and Regenerative Medicine, Stanford, CA, USA; Department of Medicine, Division of Cardiovascular Medicine, Stanford University, Stanford, CA, USA. Electronic address: smwu@stanford.edu.

Abstract

The creation of physiologically-relevant human cardiac tissue with defined cell structure and function is essential for a wide variety of therapeutic, diagnostic, and drug screening applications. Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (10⁸-10⁹ cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat frequency, and contraction-relaxation rates, suggesting their improved maturation. Our results demonstrate a novel application of Faraday waves to create stem cell-derived 3D cardiac tissue that resembles the cellular architecture of a native heart tissue for diverse basic research and clinical applications.

Keywords: Cardiac regenerative medicine; Cardiomyocytes; Human induced pluripotent stem cells; Sound wave cellular patterning.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acoustics
Bioprinting / instrumentation
Bioprinting / methods*
Cells, Cultured
Equipment Design
Humans
Induced Pluripotent Stem Cells / cytology*
Myocardium / cytology
Myocytes, Cardiac / cytology*
Sound
Tissue Engineering / instrumentation
Tissue Engineering / methods*

Abstract

Publication types

MeSH terms

Grants and funding