Molecular displaying of differential immunoresponse to various infections of amniotic epithelia

Abstract

Problem: Molecular displaying for the interaction of innate immune cells against pathogen is important for knowing their defense mechanism. This study aimed to visualize the differential gene expression profiles of amniotic epithelium, in response to various infections, using simple reverse transcription polymerase chain reaction (RT-PCR) techniques.

Method of study: Antimicrobial activity of human amniotic epithelial cells (HAECs) was verified against three different models of pathogenic microorganisms. RNA was extracted from infected and non-infected cells. Transcripts were visualized by two methods of RT-PCR; one of them targets β-defensin 2 (BDEF2). The other is a novel design method of RNA fingerprinting based on differential length amplification of transcripts (DifLAT) to polymorphism more than 69 coding sequences for antimicrobial proteins.

Results: The semiquantitative gel analysis indicated that BDEF2 was upregulated during all infections. DifLAT experiment visualized different patterns of HAECs transcripts for each case of infection.

Conclusion: This study proved that HAECs uses alternative gene expression profiles for fighting different pathogens.

Keywords: antimicrobial proteins; expression profile; innate immunity; reverse transcription.