A synthetic route to 5,8,11,14,17-eicosapentaynoic acid, an acetylenic precursor of 5Z,8Z,11Z,14Z,17Z-eicosapentaenoic acid, has been developed based on condensation of a propargyl synthon with omega-acetylenic fragments. Physico-chemical constants and spectral characteristics (IR, 1H-NMR, and mass-spectrometry) of the target and intermediate compounds are given.