Detection of Chlamydia trachomatis by in situ hybridization with sulphonated total DNA

Ann Inst Pasteur Microbiol. Jan-Feb 1988;139(1):115-27. doi: 10.1016/0769-2609(88)90099-3.

Abstract

In situ nucleic acid hybridization was applied to the detection of Chlamydia trachomatis on microscope slides by use of sulphonated total DNA as a probe. Visualization of labelled DNA was obtained using a commercial enzyme-linked monoclonal antibody. A mixture of paraformaldehyde and glutaraldehyde was found to be the best fixative. With high probe concentration (10 micrograms/ml), intracellular inclusions were detected as early as 8 h after inoculating the cell culture. Extracellular elementary bodies could also be detected. Five genital specimens were tested by in situ hybridization; the results were in agreement with those observed by culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlamydia Infections / diagnosis
  • Chlamydia trachomatis / isolation & purification*
  • Cytosine
  • DNA, Bacterial / isolation & purification*
  • Female
  • Fixatives
  • Humans
  • Inclusion Bodies / ultrastructure
  • Male
  • Nucleic Acid Hybridization
  • Sulfites

Substances

  • DNA, Bacterial
  • Fixatives
  • Sulfites
  • Cytosine
  • sodium bisulfite