Polyphosphoinositide hydrolysis in response to light stimulation of rat and chick retina and retinal rod outer segments

Biochim Biophys Acta. 1988 Jun 30;970(2):205-11. doi: 10.1016/0167-4889(88)90180-2.

Abstract

Phosphoinositides of chick and rat retina were labelled with [3H]inositol. Exposure of retinal preparations to light for 30 s caused loss of labelled phosphatidylinositol 4,5-bisphosphate and to a smaller extent of the other phosphoinositides. Similar light-induced changes were seen when rod outer segment preparations were used and, when these were illuminated in calcium-free media, phosphatidylinositol 4,5-bisphosphate was the only lipid affected. No inositol 1,4,5-trisphosphate was seen after either 30 s or 5 s of illumination of retina or 30 s illumination of rod outer segments. It is concluded that this compound plays no direct part in vertebrate photoreceptor light transduction, though phosphoinositide metabolism might relate to adaptation mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chick Embryo
  • Hydrolysis
  • Inositol Phosphates / metabolism
  • Male
  • Phosphatidylinositols / isolation & purification
  • Phosphatidylinositols / metabolism*
  • Photic Stimulation*
  • Photoreceptor Cells / analysis
  • Photoreceptor Cells / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Retina / analysis
  • Retina / metabolism*
  • Retina / physiology
  • Rod Cell Outer Segment / analysis
  • Rod Cell Outer Segment / metabolism*
  • Rod Cell Outer Segment / physiology

Substances

  • Inositol Phosphates
  • Phosphatidylinositols