AIDing-targeted protein degradation in Drosophila

FEBS J. 2017 Apr;284(8):1178-1181. doi: 10.1111/febs.14054. Epub 2017 Apr 5.

Abstract

Conditional protein depletion is highly desirable for investigating protein functions in complex organisms. In this issue, Bence and colleagues combined auxin-inducible degradation with CRISPR, establishing an elegant tool to control protein levels. They achieve precise spatio-temporal control of protein degradation during Drosophila oogenesis and early embryogenesis by combining suitable GAL4 drivers (spatial control) with auxin feeding protocols (temporal control).

Publication types

  • Comment

MeSH terms

  • Animals
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Drosophila / embryology
  • Drosophila / metabolism*
  • Embryonic Development
  • Indoleacetic Acids / metabolism*
  • Oogenesis
  • Proteins / metabolism*

Substances

  • Indoleacetic Acids
  • Proteins