Abstract
The nucleotide sequence of the gene for 10-formyltetrahydrofolate synthetase (EC 6.3.4.3) from Clostridium acidiurici ("Clostridium acidi-urici") was determined. The synthetase mRNA initiation and termination regions were determined by primer extension and S1 nuclease mapping. Two potential -10 and -35 promoter regions were identified upstream of mRNA initiation. The terminator region was found to be in a large region of dyad symmetry. A comparison of the amino acid sequences of the monofunctional synthetase and the eucaryotic trifunctional enzyme, C1-tetrahydrofolate synthase, from Saccharomyces cerevisiae demonstrated a region of strong homology.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Aminohydrolases / genetics*
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Base Sequence
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Clostridium / enzymology
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Clostridium / genetics*
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Codon / genetics
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Endonucleases
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Formate-Tetrahydrofolate Ligase / genetics*
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Genes, Bacterial
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Genes, Fungal
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Ligases / genetics*
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Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics*
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Molecular Sequence Data
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Multienzyme Complexes / genetics*
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Nucleic Acid Hybridization
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Oxidoreductases / genetics*
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Saccharomyces cerevisiae / genetics*
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Sequence Homology, Nucleic Acid
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Single-Strand Specific DNA and RNA Endonucleases
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Transcription, Genetic
Substances
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Codon
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Multienzyme Complexes
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RNA, Messenger
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formyl-methenyl-methylenetetrahydrofolate synthetase
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Oxidoreductases
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Methylenetetrahydrofolate Dehydrogenase (NADP)
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Endonucleases
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Single-Strand Specific DNA and RNA Endonucleases
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Aminohydrolases
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Ligases
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Formate-Tetrahydrofolate Ligase