The Escherichia coli Ras-like protein (Era) has GTPase activity and is essential for cell growth

Oncogene. 1988 Jun;2(6):539-44.


The era gene of Escherichia coli encodes a protein (Era) which is similar to the eucaryotic RAS family of proteins. We report here that purified Era possesses both GTP-binding and GTPase activities. Era is also shown to be loosely associated with the inner membrane of E. coli. Overproduction of Era to 5% of the total cellular protein does not apparently alter either cell growth or cAMP levels. Disruption of the era gene by insertional inactivation is shown to be lethal by construction of a conditional lethal era mutant strain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology*
  • Cell Division*
  • Cell Membrane / physiology
  • Cloning, Molecular
  • DNA Mutational Analysis
  • Escherichia coli / physiology
  • GTP Phosphohydrolases / physiology*
  • GTP-Binding Proteins / physiology*
  • Genes, Bacterial
  • Genes, Lethal
  • Membrane Proteins / physiology
  • Phosphoric Monoester Hydrolases / physiology*


  • Bacterial Proteins
  • Membrane Proteins
  • Phosphoric Monoester Hydrolases
  • GTP Phosphohydrolases
  • GTP-Binding Proteins