Mycobacterium tuberculosis lysine-ɛ-aminotransferase a potential target in dormancy: Benzothiazole based inhibitors

Bioorg Med Chem. 2017 May 15;25(10):2761-2771. doi: 10.1016/j.bmc.2017.03.053. Epub 2017 Mar 27.

Abstract

MTB lysine-ɛ-aminotransferase (LAT) was found to play a crucial role in persistence and antibiotic tolerance. LAT serves as a potential target in the management of latent tuberculosis. In present work we attempted to derivatize the benzothiazole lead identified through high throughput virtual screening of Birla Institute of Technology and Science in house database. For Structure activity relationship purpose 22 derivatives were synthesized and characterized. Among synthesized compounds, eight compounds were found to be more efficacious in terms of LAT inhibition when compared to lead compound (IC50 10.38±1.21µM). Compound 22 exhibits bactericidal action against nutrient starved Mycobacterium tuberculosis (MTB). It also exhibits significant activity in nutrient starvation model (2.9log folds) and biofilm model (2.3log folds).

Keywords: 3D granuloma model; Benzothiazole derivatives; Dormancy; Lysine-ɛ amino transferase; Mycobacterium tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / chemistry*
  • Antitubercular Agents / metabolism
  • Antitubercular Agents / pharmacology
  • Bacterial Proteins / antagonists & inhibitors*
  • Bacterial Proteins / metabolism
  • Benzothiazoles / chemistry*
  • Benzothiazoles / metabolism
  • Benzothiazoles / pharmacology
  • Binding Sites
  • Catalytic Domain
  • Drug Design
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology
  • Molecular Docking Simulation
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / metabolism*
  • Structure-Activity Relationship
  • Transaminases / antagonists & inhibitors*
  • Transaminases / metabolism

Substances

  • Antitubercular Agents
  • Bacterial Proteins
  • Benzothiazoles
  • Enzyme Inhibitors
  • Transaminases