Isolation and identification of multiple drug resistant nontuberculous mycobacteria from organs of cattle produced typical granuloma lesions

Guangyu Cheng; Donglei Xu; Jinling Wang; Chunfa Liu; Yang Zhou; Yongyong Cui; Hancan Liu; Kanglin Wan; Xiangmei Zhou

doi:10.1016/j.micpath.2017.03.047

Isolation and identification of multiple drug resistant nontuberculous mycobacteria from organs of cattle produced typical granuloma lesions

Microb Pathog. 2017 Jun:107:313-316. doi: 10.1016/j.micpath.2017.03.047. Epub 2017 Apr 6.

Authors

Guangyu Cheng¹, Donglei Xu², Jinling Wang³, Chunfa Liu⁴, Yang Zhou⁵, Yongyong Cui⁶, Hancan Liu⁷, Kanglin Wan⁸, Xiangmei Zhou⁹

Affiliations

¹ National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: hnchdd@163.com.
² National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Tuberculosis Reference Laboratory, Changping, 100193 Beijing, China. Electronic address: xudonglei@icdc.cn.
³ College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018 Huhhot, China. Electronic address: wangjinlin-721@163.com.
⁴ National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: 75636301@qq.com.
⁵ National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: 79554071@qq.com.
⁶ National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: 569057286@qq.com.
⁷ National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Tuberculosis Reference Laboratory, Changping, 100193 Beijing, China. Electronic address: liuhaican@icdc.cn.
⁸ National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, National Tuberculosis Reference Laboratory, Changping, 100193 Beijing, China. Electronic address: wankanglin@icdc.cn.
⁹ National Animal Transmissible Spongiform Encephalopathy Laboratory, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Lab of Agrobiotechnology, China Agricultural University, 100193 Beijing, China. Electronic address: zhouxm@cau.edu.cn.

PMID: 28392413
DOI: 10.1016/j.micpath.2017.03.047

Abstract

Nontuberculosis mycobacteria are widespread in the environment and some are zoonotic. 320 tissue samples with visible lesions were obtained from dairy cows and examined by histopathology. Eleven samples showed typical granulomatous lesions and a total of 8 strains were cultured. Three genes (16S rRNA, hsp65 and rpoB) were sequenced for species identification. All mycobacterial isolates were tested for rifampicin, isoniazid, ethambutol, streptomycin, capreomycin, kanamycin, para-aminosalicylic acid susceptibility. Six strains were identified as M. fortuitum, 1 was M. avium, 1 was M. conceptionense, isolated from cattle for the first time. Seven of the 8 isolated strains showed multiple drug resistance.

Keywords: Drug resistance; Histopathology; M. fortuitum; Typical granulomatous lesions.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Cattle
Cattle Diseases / microbiology
Chaperonin 60 / genetics
China
DNA, Bacterial
DNA-Directed RNA Polymerases / genetics
Drug Resistance, Multiple, Bacterial*
Genes, Bacterial / genetics
Granuloma / microbiology*
Granuloma / pathology
Liver / pathology
Microbial Sensitivity Tests
Mycobacterium Infections / microbiology*
Mycobacterium Infections / pathology
Necrosis / microbiology
Necrosis / pathology
Nontuberculous Mycobacteria / classification
Nontuberculous Mycobacteria / drug effects
Nontuberculous Mycobacteria / genetics
Nontuberculous Mycobacteria / isolation & purification*
RNA, Ribosomal, 16S / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
Chaperonin 60
DNA, Bacterial
RNA, Ribosomal, 16S
heat-shock protein 65, Mycobacterium
rpoB protein, Mycobacterium tuberculosis
DNA-Directed RNA Polymerases