Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity

J Vet Med Sci. 2017 May 18;79(5):896-903. doi: 10.1292/jvms.17-0083. Epub 2017 Apr 8.


Hyperoxaluria and oxidative stress are risk factors in calcium oxalate (CaOx) stone formation. Supplement with antioxidant could be effective in prevention of recurrent stone formation. The present study aims to evaluate the protective effects of vitamin E and vitamin C in hyperoxaluric rat. The experiment was performed in rats for 21 days. Rats were divided into 5 groups as follows: control (group 1, n=8), hyperoxaluric rats (group 2, n=8), hyperoxaluric rats with vitamin E supplement (group 3, n=7), hyperoxaluric rats with vitamin C supplement (group 4, n=7) and hyperoxaluric rats with vitamin E and C supplement (group 5, n=7). Hyperoxaluria was induced by feeding hydroxyl L-proline (HLP) 2% w/v dissolved in drinking water. Intraperitoneal 200 mg/kg of vitamin E was given in groups 3 and 5 on days 1, 6, 11 and 16, while 500 mg of vitamin C was injected intravenously in groups 4 and 5 on days 1 and 11. Renal functions and oxidative status were measured. The urinary oxalate excretion was increased in HLP supplement rats, while glomerular filtration rate, proximal water and sodium reabsorption were significantly lower in group 2 compared with a control (P<0.05). Giving antioxidants significantly lower urinary calcium oxalate crystals (P<0.05). Hyperoxaluric rats had higher plasma malondialdehyde (PMDA) and lower urinary total antioxidant status (UTAS), which were alleviated by vitamin E and/or vitamin C supplement. In conclusion, giving combination of vitamin E and vitamin C exerts a protective role against HLP-induced oxalate nephropathy.

Keywords: glomerular filtration rate; hyperoxaluria; oxidative stress; vitamin C; vitamin E.

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / therapeutic use*
  • Ascorbic Acid / administration & dosage
  • Ascorbic Acid / therapeutic use*
  • Body Weight
  • Citrates / urine
  • Drinking
  • Drug Therapy, Combination
  • Eating
  • Electrolytes / metabolism
  • Hemodynamics
  • Hyperoxaluria / drug therapy*
  • Hyperoxaluria / pathology
  • Kidney / drug effects*
  • Kidney / pathology
  • Kidney Calculi / prevention & control
  • Kidney Glomerulus / drug effects
  • Kidney Glomerulus / physiology
  • Male
  • Oxalates / urine
  • Protective Agents / therapeutic use
  • Rats
  • Rats, Sprague-Dawley
  • Vitamin E / administration & dosage
  • Vitamin E / therapeutic use*


  • Antioxidants
  • Citrates
  • Electrolytes
  • Oxalates
  • Protective Agents
  • Vitamin E
  • Ascorbic Acid