SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene

Cell. 1988 Jul 15;54(2):275-83. doi: 10.1016/0092-8674(88)90559-4.


Monkey cells synthesizing SV40 large T antigen were lysed and the extracts immunoprecipitated with either monoclonal anti-T antibody or monoclonal antibody to p110-114, the product of the retinoblastoma susceptibility gene (Rb). T and p110-114 coprecipitated in each case, implying that the proteins are complexed with each other. Substitution and internal deletion mutants of T that contain structural alterations in a ten residue, transformation-controlling domain failed to complex with p110-114. In contrast, T mutants bearing structural changes outside of this domain bound to p110-114. These results are consistent with a model for transformation by SV40 which, at least in part, involves T/p110-114 complex formation and the perturbation of Rb protein and/or T function.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Polyomavirus Transforming* / genetics
  • Antigens, Polyomavirus Transforming* / immunology
  • Cell Line
  • Disease Susceptibility
  • Electrophoresis, Polyacrylamide Gel
  • Eye Neoplasms / genetics*
  • Haplorhini
  • Immunoassay
  • Mutation
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / immunology
  • Neoplasm Proteins / metabolism
  • Phosphoproteins / genetics*
  • Phosphoproteins / immunology
  • Phosphoproteins / metabolism
  • Retinoblastoma / genetics*
  • Simian virus 40 / genetics
  • Transfection


  • Antibodies, Monoclonal
  • Antigens, Polyomavirus Transforming
  • Neoplasm Proteins
  • Phosphoproteins