Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview

Biopreserv Biobank. 2017 Aug;15(4):305-309. doi: 10.1089/bio.2016.0105. Epub 2017 Feb 22.


The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.

Keywords: PBMCs; SOP; biobanks; cryopreservation; functionality.

MeSH terms

  • Biological Specimen Banks*
  • Cell Separation / methods*
  • Cell Survival
  • Cryopreservation / methods*
  • Freezing
  • Humans
  • Leukocytes, Mononuclear / cytology*
  • Spain