Purification of transiently transfected cells by magnetic affinity cell sorting

Anal Biochem. 1988 May 1;170(2):341-8. doi: 10.1016/0003-2697(88)90640-9.


A method was developed to purify transiently transfected HeLa cells or African green monkey kidney CV-1 cells by magnetic affinity cell sorting. Monolayer cultures were transfected with mammalian expression vectors coding for either of two novel cell surface antigens, the Tac subunit of the human IL-2 receptor or vesicular stomatitis virus G protein. During the transient expression phase, cell populations were placed in suspension and mixed with monoclonal-antibody-coated magnetic particles in the presence of a sorting solution designed to minimize nonspecific cell/cell and cell/particle interactions. Transfected cells expressing the vector-encoded cell surface antigen were then isolated by application of a magnetic field. Reconstruction experiments indicated that IL-2 receptor-positive cells were bound about 100-fold more efficiently than receptor-negative cells. In transient transfection experiments, populations of greater than 90% antigen-positive cells were reproducibly obtained.

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Cell Separation / methods*
  • Cells, Cultured
  • Chlorocebus aethiops
  • DNA, Recombinant
  • Gene Expression Regulation
  • Genetic Vectors
  • HeLa Cells
  • Humans
  • Kidney
  • Magnetics
  • Nerve Tissue Proteins / genetics
  • Receptors, Immunologic / genetics
  • Receptors, Interleukin-2
  • Transcription, Genetic
  • Transfection*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Viral Proteins / genetics


  • Antigens, Surface
  • DNA, Recombinant
  • G-substrate
  • Nerve Tissue Proteins
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Viral Proteins