Transposon mutagenesis in Bordetella avium

Am J Vet Res. 1988 May;49(5):687-92.

Abstract

Transposon mutagenesis was used to introduce specific mutations in the chromosome of Bordetella avium. The transposon Tn5 was transferred by conjugation to B avium from Escherichia coli strain SM10 by the suicide plasmid vector pSUP1011. A defined minimal medium for growth of B avium was developed for scoring transposon-containing mutants for specific nutritional requirements. Approximately 1% of all mutants tested were auxotrophic in some form, and these organisms were grouped into 13 classes. Also, 2 classes of hemagglutination-negative mutants were isolated. Stability of Tn5 insertion was examined, and frequencies of reversion less than 10(-7) were observed, indicating stable mutations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bordetella / genetics*
  • Conjugation, Genetic
  • DNA Transposable Elements*
  • DNA, Bacterial / analysis
  • Electrophoresis, Agar Gel
  • Escherichia coli / genetics
  • Mutation*

Substances

  • DNA Transposable Elements
  • DNA, Bacterial