Effect of aminoglycoside concentration on reaction rates of aminoglycoside-modifying enzymes

Antimicrob Agents Chemother. 1988 May;32(5):740-6. doi: 10.1128/AAC.32.5.740.

Abstract

Reaction rates of several reference aminoglycoside-modifying enzymes were studied at various substrate concentrations. The resulting concentration-response curves showed wide variation in threshold concentration, in curve slope, in enzyme saturation, and in substrate inhibition. Together, the curves of a defined aminoglycoside panel yielded more specific information for each individual aminoglycoside-modifying enzyme tested than did conventional substrate profiles obtained at a single substrate concentration.

MeSH terms

  • Acetylation
  • Acetyltransferases / metabolism*
  • Aminoglycosides
  • Anti-Bacterial Agents / metabolism*
  • Bacteria / enzymology*
  • Escherichia coli / enzymology
  • Kanamycin Kinase
  • Moraxella / enzymology
  • Nucleotidyltransferases / metabolism*
  • Phosphorylation
  • Phosphotransferases / metabolism*
  • Providencia / enzymology
  • Pseudomonas aeruginosa / enzymology
  • Staphylococcus aureus / enzymology

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Acetyltransferases
  • aminoglycoside acetyltransferase
  • Phosphotransferases
  • Kanamycin Kinase
  • Nucleotidyltransferases
  • gentamicin 2''-nucleotidyltransferase