Prevalence and genotype distribution of hepatitis delta virus among chronic hepatitis B carriers in Central Vietnam

PLoS One. 2017 Apr 12;12(4):e0175304. doi: 10.1371/journal.pone.0175304. eCollection 2017.


Hepatitis D virus (HDV) infection plays an important role in liver diseases. However, the molecular epidemiology and impact of HDV infection in chronic hepatitis B (CHB) remain uncertain in Vietnam. This cross-sectional study aimed to investigate the prevalence and genotype distribution of HDV among HBsAg-positive patients in Central Vietnam. 250 CHB patients were tested for HDV using newly established HDV-specific RT-PCR techniques. HDV genotypes were determined by direct sequencing. Of the 250 patients 25 (10%) had detectable copies of HDV viral RNA. HDV-2 was predominant (20/25; 80%) followed by HDV-1 (5/25; 20%). Proven HDV genotypes share the Asian nomenclature. Chronic hepatitis B patients with concomitant HDV-1 showed higher HBV loads as compared to HDV-2 infected patients [median log10 (HBV-DNA copies/ml): 8.5 vs. 4.4, P = 0.036]. Our findings indicate that HDV infection is highly prevalent and HDV-2 is predominant in Central Vietnam. The data will add new information to the management of HBsAg-positive patients in a highly HBV endemic region to in- or exclude HDV infection in terms of diagnostic and treatment options.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Coinfection / epidemiology*
  • Coinfection / virology
  • Cross-Sectional Studies
  • Female
  • Genotype
  • Hepatitis B virus / genetics
  • Hepatitis B virus / isolation & purification*
  • Hepatitis B, Chronic / epidemiology*
  • Hepatitis B, Chronic / virology
  • Hepatitis D / epidemiology*
  • Hepatitis D / virology
  • Hepatitis Delta Virus / genetics
  • Hepatitis Delta Virus / isolation & purification*
  • Humans
  • Male
  • Middle Aged
  • Phylogeny
  • Prevalence
  • Vietnam / epidemiology
  • Young Adult

Grant support

This work was sponsored by Vietnam’s National Foundation for Science and Technology Development (NAFOSTED) grant no. 106-YS.02-2014.03 and hepatitis international delta network (HDIN). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.