Standardization of biopolymer production from seaweed associative bacteria

Int J Biol Macromol. 2017 Sep;102:550-564. doi: 10.1016/j.ijbiomac.2017.04.032. Epub 2017 Apr 9.

Abstract

Polyhydroxyalkanoate (PHA) is the biodegradable plastic, which is shown the similar properties to the synthetic plastic. Production of PHA is expensive when compared with petrochemical based plastics costs and also hazardous to ecosystem. In order to overcome these drawbacks, some cheaper commercially available carbon sources like rice bran, coconut cake, palm jaggary, etc. can be used. The present study aimed to identify the potential PHA producing bacterial strains from different seaweeds. Based on PHA production and crotonic acid assay, two bacterial strains were screened and designated as M3 and S6 which were found to be efficient PHA producers. Potential PHA producers of M3 and S6 were identified as Bacillus cereus RBL6 and Pseudomonas pseudoalcaligens RBL7 by 16S rRNA gene sequencing. The physical and nutritional parameters such as pH, temperature, incubation period, substrate, carbon and nitrogen concentration have been used for enhancing PHA production which was served as precursor. The purified PHA had been chemically characterized by FT-IR, GC-MS and viewed through SEM.

Keywords: Bacillus cereus RBL6; Biopolymer; FT-IR; GC–MS; PHA; Pseudomonas pseudoalcaligens RBL7; SEM; Seaweed associated bacteria.

MeSH terms

  • Bacillus cereus / genetics
  • Bacillus cereus / isolation & purification
  • Bacillus cereus / metabolism*
  • Biopolymers / biosynthesis*
  • Biotechnology / standards*
  • Phylogeny
  • Pseudomonas / genetics
  • Pseudomonas / isolation & purification
  • Pseudomonas / metabolism*
  • RNA, Ribosomal, 16S / genetics
  • Reference Standards
  • Seaweed / microbiology*

Substances

  • Biopolymers
  • RNA, Ribosomal, 16S