Activation of T cell-derived lymphokine genes in T cells and fibroblasts: effects of human T cell leukemia virus type I p40x protein and bovine papilloma virus encoded E2 protein

Nucleic Acids Res. 1988 Jul 25;16(14A):6547-66. doi: 10.1093/nar/16.14.6547.


The effects of p40x, a product of an human T cell leukemia virus type I, on the activation of lymphokine genes were examined. The mouse GM-CSF and IL-3 genes were activated by cotransfection with a pX containing plasmid both in Jurkat and CV1 cells. Mouse GM-CSF gene was also activated by phytohaemagglutinin A (PHA)/phorbol myristate acetate (PMA) or PMA/calcium ionophore A23187 stimulation. The 5'-flanking region of the mouse GM-CSF gene which is required for activation by pX or mitogen was mapped within 226 bp upstream from the transcription initiation site. Action of pX was not restricted to T cells. pX activated exogenously added GM-CSF, IL-2, IL-3 and IL-4 genes in fibroblasts. Activation of the GM-CSF gene in fibroblasts appears to require the same regulatory region as in T cells. Similar results were obtained using bovine papilloma virus encoded E2 protein. We propose that pX or E2 protein, both in T cells and fibroblasts, activates cellular component(s) in the signal transduction pathway which results in the activation of lymphokine genes in the absence of extracellular stimuli.

MeSH terms

  • Adenovirus Early Proteins
  • Animals
  • Bovine papillomavirus 1 / physiology*
  • Cell Line
  • Colony-Stimulating Factors / genetics
  • DNA-Binding Proteins / physiology
  • Deltaretrovirus / physiology*
  • Fibroblasts / physiology*
  • Gene Expression Regulation
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Growth Substances / genetics
  • Humans
  • Interleukin-2 / physiology
  • Interleukin-3 / physiology
  • Lymphokines / physiology*
  • Oncogene Proteins, Viral / physiology
  • Papillomaviridae / physiology*
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
  • Retroviridae Proteins / physiology*
  • T-Lymphocytes / physiology*
  • Transcription Factors / physiology*
  • Viral Proteins / physiology


  • Adenovirus Early Proteins
  • Colony-Stimulating Factors
  • DNA-Binding Proteins
  • E2 protein, Bovine papillomavirus
  • Growth Substances
  • Interleukin-2
  • Interleukin-3
  • Lymphokines
  • Oncogene Proteins, Viral
  • Retroviridae Proteins
  • Transcription Factors
  • Viral Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor