Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

Methods Mol Biol. 2017:1595:63-67. doi: 10.1007/978-1-4939-6937-1_7.


Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts.

Keywords: Functional complementation; Nucleofection; Primary cells; Transfection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fibroblasts / metabolism*
  • Humans
  • Peroxisomes / metabolism*
  • Primary Cell Culture
  • Skin / cytology*
  • Transfection*