Analysis of Peroxisomal β-Oxidation During Storage Oil Mobilization in Arabidopsis thaliana Seedlings

Methods Mol Biol. 2017;1595:291-304. doi: 10.1007/978-1-4939-6937-1_27.

Abstract

Peroxisomal β-oxidation in plants is essential for mobilization of storage oil in seed-oil storing plants, such as Arabidopsis thaliana. In plants, degradation of fatty acids occurs exclusively in peroxisomes via β-oxidation, driving seedling growth and development upon germination. Thus, the determination of storage oil breakdown rates is a useful approach to investigate defects in peroxisomal β-oxidation. Here we describe an acid catalyzed derivatization process of fatty acids representing a fast and efficient procedure to generate high yields of fatty acid methyl esters (FAMEs). The subsequent analysis by gas chromatography coupled to mass spectrometry (GC-MS) allows the quantification of total fatty acid content. The results provide detailed information of the complete storage oil breakdown process via peroxisomal β-oxidation during seedling growth.

Keywords: Fatty acid β-oxidation; Peroxisomes; Plant seedling; Storage oil mobilization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Fatty Acids / metabolism
  • Gas Chromatography-Mass Spectrometry
  • Germination
  • Oxidation-Reduction*
  • Peroxisomes / metabolism*
  • Plant Extracts
  • Plant Oils / metabolism*
  • Seedlings / metabolism*
  • Seeds / growth & development
  • Seeds / metabolism

Substances

  • Fatty Acids
  • Plant Extracts
  • Plant Oils