Progressive loss of DNA sequences from terminal chromosome deficiencies in Drosophila melanogaster

EMBO J. 1988 Apr;7(4):1081-6.


Terminal deficiencies at the tip of the X chromosome can be induced at a high frequency (0.2-0.3%) by irradiating Drosophila females carrying a homozygous mutator (mu-2) with low doses of X-rays. These terminal deficiencies are unstable, since over a period of 3 1/2 years DNA sequences were lost from their distal ends at a rate of 75 bp per generation, presumably due to the absence of a complete wild-type telomeric structure. Breakpoints of these deletions in the 5' upstream regulatory region of the yellow gene, giving rise to a mosaic cuticle pigmentation pattern typical of the y2 type, were used to define the location of tissue-specific cis-acting regulatory elements that are required for body, wing or bristle pigmentation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromosomes / physiology
  • Crosses, Genetic
  • DNA / genetics*
  • DNA / radiation effects
  • DNA Restriction Enzymes
  • Drosophila melanogaster / genetics*
  • Female
  • Genes, Regulator
  • Male
  • Mutation*
  • X-Rays


  • DNA
  • DNA Restriction Enzymes