Effect of exogenously added acylphosphatases on inositol lipid metabolism in human platelets

FEBS Lett. 1988 Aug 1;235(1-2):229-32. doi: 10.1016/0014-5793(88)81268-7.

Abstract

In this paper we demonstrate that human platelets contain an acylphosphatase isoenzyme. We then investigated the effect of exogenously added human muscle and erythrocyte acylphosphatases on inositol lipid content in human platelets permeabilized with saponin. Alterations in the level of the polyphosphoinositides were observed: in particular, the levels of phosphatidylinositol 4,5-bisphosphate, and of phosphatidylinositol 4-monophosphate were decreased, whereas the level of phosphatidylinositol was increased. These results suggest that acylphosphatases promote polyphosphoinositide dephosphorylation, possibly through intracellular Ca2+ mobilization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Anhydride Hydrolases*
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism*
  • Calcium / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Erythrocytes / enzymology
  • Humans
  • Isoenzymes / blood
  • Isoenzymes / pharmacology*
  • Muscles / enzymology
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositol Phosphates*
  • Phosphatidylinositols / blood*
  • Phosphoric Monoester Hydrolases / blood
  • Phosphoric Monoester Hydrolases / pharmacology*

Substances

  • Isoenzymes
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositol Phosphates
  • Phosphatidylinositols
  • phosphatidylinositol 4-phosphate
  • Phosphoric Monoester Hydrolases
  • Acid Anhydride Hydrolases
  • acylphosphatase
  • Calcium