Gas chromatography mass spectrometry (GC-MS) based metabolomics strategy was implemented for the metabolites detection in kidney and spleen samples of mice, which were treated with 2 mg kg-1 deoxynivalenol (DON), through intragastric administration for three weeks, for studying the toxicity of DON on the metabolic profiling in kidney and spleen. The spectrum was deconvoluted, aligned and identified with MS DIAL, equipped with Fiehn library. And the data matrix was processed with univariate analysis and multivariate analysis for selection of metabolites with VIP >1, t-test p value < 0.05. The metabolic pathway analysis was analyzed with MetaMapp and drew by CytoScape. Result shows that DON could induce an increased protein synthesis to repair the damaged membrane protein structure, in both kidney and spleen, with decrease of valine, leucine and phenylalanine, et al. essential precursors for protein synthesis and energy production; the energy metabolism in kidney disordered by DON, with the decreasing of ribitol, glycerol 1-phosphate, et al. Furthermore, DON could lead to the disorder in immunity function and nucleotide metabolism in spleen, with decreasing trend of cytidine and alanine.
Keywords: Deoxynivalenol; Kidney toxicity; Metabolomics; Spleen toxicity.
Copyright © 2017. Published by Elsevier Ltd.